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SIELC
SIELC HPLC Column SHARC 1, 100 Å, 5 μm, 22 x 50 mm
SHARC columns are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Chromatography. The SHARC 1 column is the first of the SHARC series columns. Hydrogen bonding is an interaction between hydrogen atom bound to electronegative atoms in a molecule, such as oxygen, nitrogen, fluorine. This is typically a weak interaction, especially when separation is performed in aqueous solutions. Liquid chromatography techniques evolved as tool for separation of different molecules based on their physico-chemical properties. Most common techniques of the separation are: Hydrophobic separation based on degree of hydrophobicity of the molecule Ion-exchange separation based on number, nature, and distribution of charges in the molecule. Normal phase separation based on hydrophilic properties of the molecules including molecule dipoles value and dipoles position SShow more
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